Electron Microscopy - Immunogold labelling is the most widely used methodology in electron microscope Immunocytochemistry. Gold labels are very electron-dense and can be readily distinguished from cellular components.
Light Microscopy - Immunogold staining in combination with silver enhancement is a rapid, sensitive technique which permits very precise localization of antigen compared to enzymatic detection methods.
Blotting - Immunogold conjugates may be used with silver enhancement to visualize DNA, RNA or proteins on nitrocellulose, PVDF or nylon membranes
“In Situ” Hybridization - Specific DNA and RNA sequences can be readily be identified using gold labelling after in situ” Hybridization with biotinylated or digoxigenin labelled DNA probes.