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Embedding Media Kits

Embedding Media Kits

 

Canemco Inc. offers a wide range of embedding media kits. Each kit contains, in addition to it's chemical components, a short set of suggested instructions on how to prepare various formulas. In general it is essential to mix the embedding components very well to obtain a uniform polymerization. Poor mixing is one of the main causes of sectioning problems, especially with epoxy resins.


Note: When working with any embedding components or chemicals always wear appropriate protective gear, work under a fume hood where applicable and observe all recommended handling and safety procedures.



JEMBED 812 Embedding Kit

JEMBED 812 embedding kitJEMBED 812 resin produces polymerized blocks which are highly stable in the electron  beam and do not exhibit the shrinkage problems associated with Methacrylate embedding. The hardness of the blocks can be varied over a wide range by altering the ratio of the two hardeners used.

 

 

 

JEMBED 812 Embedding Kit

Kit Contents

Qty

JEMBED 812 resin

500 g

DDSA, specially distilled

250 ml

NMA

250 ml

DMP-30

 25 ml

 

Catalog #

Description

Qty

050

JEMBED 812 embedding kit

1 kit

Please use the catalog number and description when ordering.


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JEMBED - Araldite Kit


Excellent cutting, preservation and staining properties. The hardness may be varied.

 

JEMBED - Araldite Kit

Kit Contents

Qty

JEMBED 812 resin

500 g

Araldite 502 resin

450 ml

DDSA, specially distilled

2 x 250 ml

DMP-30

25 ml


 

Catalog #

Description

Qty

0501

JEMBED-Araldite embedding kit

1 kit

Please use the catalog number and description when ordering.

 

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Polyethylene Cups and caps are very useful

 for mixing and storing resins.

Please see our complete line of Laboratory Plastic ware

 

 

 


 

Araldite 502 Resin Kit

 

Araldite resin produces relatively hard blocks with minimal shrinkage allowing the easy production of thin sections. The use of an intermediate solvent such as propylene oxide allows rapid infiltration of the specimen by this high viscosity resin.

 

Araldite 502 Resin Kit

Kit Contents

Qty

Araldite 502 resin

450 ml

DDSA, specially distilled

250 ml

DMP-30

25 ml

 

 

Catalog #

Description

Qty

051

Araldite 502 embedding kit

1 kit

Please use the catalog number and description when ordering.


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Araldite 6005 Embedding Kit

 

Araldite 6005 resin produces slightly harder blocks than  Araldite 502 resin

 

Araldite 6005 Embedding Kit

Kit Contents

Qty

Araldite 6005 resin

450 ml

DDSA, specially distilled

250 ml

DBP

225 ml

BDMA

25 ml

 

Catalog #

Description

Qty

05101

Araldite 6005 embedding kit

1 kit

Please use the catalog number and description when ordering.

 

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Low Viscosity Resin Kit

As reported by Dr.Spurr
Spurr, A.R.,(1969) J.Ultrastruct. Res. 26, 31-42

Low viscosity resin kit


Spurr VCD resin gives exceptionally good penetration results due to its very low viscosity. It  has good cutting characteristics aided by the block surface being hydrophobic. It also has the advantage of being more transparent than most epoxy resins. The hardness is adjusted by changing the proportion of the DER-736.

 

Low Viscosity Resin Kit

Kit Contents

Qty

NSA, specially distilled

450 m

DER-736

225 ml

ERL 4206 (VCD)

 225 ml

DMAE

 25 ml

 

Catalog #

Description

Qty

052

Low viscosity resin kit

1 kit

Please use the catalog number and description when ordering.

 

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Vestopal 310 Embedding Kit
(Formerly Vestopal W)


Vestopal 310 is a styrene-polyester based resin of high viscosity. Its polymer is fully cross linked and therefore very stable in the electron beam. This resin has a fine grain, sections easy and stains well. It hardens without shrinkage.
 

Vestopal 310 Embedding Kit

Kit Contents

Qty

Vestopal 310

 450 ml

Cobalt napthenate

10 ml

t-Butyl perbenzoate

25 ml

 

Catalog #

Description

Qty

053

Vestopal 310 embedding kit

1 kit

Please use the catalog number and description when ordering.


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TAAB 812 Resin Kit


A high quality resin produced in small batches. It has a high specification, and a weight per Epoxide (WPE) of 148-150.   It is a reliable, popular resin suitable for EM and giving very good results in LM, but it still has quite a high viscosity. While this is acceptable for EM, it can restrict specimen size for LM.
 

TAAB 812 Resin Kit

Kit Contents

Qty

TAAB 812 resin

 500 g

DDSA, EM

250 ml

NMA

250 ml

DMP-30

25 ml

 

Catalog #

Description

Qty

056

TAAB 812 resin kit

1 kit

Please use the catalog number and description when ordering.


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TAAB Embedding Resin Kit

 

A relatively low viscosity epoxy resin developed by TAAB to counter some of the deficits of both Araldite and Epon 812. Developed for biological specimens for EM and LM, it exhibits excellent cutting and staining qualities, with freedom from background “grain”. A wide range of hardness can be obtained by varying the proportions of the hardeners DDSA and NMA.

 

TAAB Embedding Resin Kit

Kit Contents

Qty

TAAB embedding resin

500 g

DDSA, EM

250 g

NMA

250 g

DMP-30

25 ml

 

Catalog #

Description

Qty

0561

TAAB embedding resin kit

1 kit

Please use the catalog number and description when ordering.


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GMA-Glycol Methacrylate Kit


Water soluble embedding kit. Glycol Methacrylate embedding medium for excellent preservation (especially enzymatic autoradiographic studies). Good for EM, LM and Histological applications.
 

GMA- Glycol Methacrylate Kit

Kit Contents

Qty

GMA-Glycol Methacrylate

450 ml

n-Butylmethacrylate

225 ml

Benzoyl Peroxide paste

15 g

 

Catalog #

Description

Qty

054

GMA-Glycol Methacrylate embedding kit

1 Kit

Please use the catalog number and description when ordering.

 

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Transmit Resin

 

TAAB Transmit EM resin kit


A low viscosity epoxy resin with comparable performance to Spurr’s resin, except that a softer block is usually produced. It has good sectioning characteristics, and electron beam stability. It is a good resin for LM use and is easy to handle. An exceptional advantage is that the monomer is water miscible and can be used to dehydrate specimens where alcohol and acetone should be avoided.


Two kits are available

 

Transmit  LM  Kit

Kit Contents

Qty

 

The Transmit LM Kit is designed for semi-thin sectioning for Light Microscopy.
 

Transmit LM resin

3 x 100 g

Transmit hardener TH1

8 x 100 g

Accelerator

1 x 25 ml

 

Transmit  EM  Kit

Kit Contents

Qty

 

The Transmit EM Kit is designed for ultra-thin sectioning for Electron Microscopy
 

Transmit EM resin

3 x 100 g

Transmit hardener TH1

5x 100 g

Transmit hardener TH2

3 x 100 g

Accelerator

1 x 25 ml

 

Catalog #

Description

Qty

0602

TAAB Transmit LM resin kit

1 kit

0603

TAAB Transmit EM resin kit

1 kit

Please use the catalog number and description when ordering.


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Quetol 651 - NSA Embedding Kit


Quetol 651 is a low viscosity, water miscible epoxy resin. The kit is light colored and affords easy infiltration, embedding, sectioning and staining for both LM and EM.
 

Quetol 651 - NSA Embedding Kit

Kit Contents

Qty

Quetol 651

225 ml

NMA

450 ml

NSA

450 ml

DMP-30

50 ml

 

Catalog #

Description

Qty

062

Quetol 651 - NSA embedding kit

1 Kit

Please use the catalog number and description when ordering.

 

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TAAB Premix Kit

 

Taab 812 Premix Kit


TAAB has available in PREMIX form the popular embedding resins (Spurr’s, and Taab 812) with which the microscopist is already fully familiar and obtains high quality results. The PREMIX offers the added bonus of increased reproducibility of results as weighing of components is no longer necessary.


The procedure is very simple:


Each kit consists of 5 sets of each of the two components, plus 5 ampoules of accelerator. One component is in a 125 ml capacity bottle, the other is in a 60 ml capacity bottle.


Simply empty the contents from the smaller bottle into the larger bottle, mix, then add the contents of one ampoule of accelerator, mix thoroughly again and approximately 100g  of resin media is now ready for use. No additional containers are required, and any unused resin can either be stored in a refrigerator or freezer for later use or left to set in the bottle before it is discarded.
 

 

Spurr Premix Resin Kits

Hard

Resin Kit

Kit Contents

Qty

Resin-hard

5 x 35g

Hardener

5 x 65g

Accelerator

5 x 1ml

Medium Resin Kit

Kit Contents

Qty

Resin-medium

5 x 35g

Hardener

5 x 65g

Accelerator

5 x 1ml

Soft

Resin Kit

Kit Contents

Qty

Resin-soft

5 x 35g

Hardener

5 x 65g

Accelerator

5 x 1ml

 

Catalog #

Description

Qty

065

 Spurr Premix Hard resin kit

1 Kit

0651

Spurr Premix Medium resin kit

1 Kit

0652

Spurr Premix Soft resin kit

1 Kit

Please use the catalog number and description when ordering.

 

TAAB 812 Premix Resin Kits

TAAB

Premix

Hard

Kit Contents

Qty

Resin

5 x 48g

Hardener HARD

5 x 52g

Accelerator

5 x 2.5ml

TAAB

Premix Medium 

Kit Contents

Qty

Resin

5 x 48g

Hardener MEDIUM

5 x 52g 

Accelerator

5 x 2.5ml

TAAB

Premix

Soft

Kit Contents

Qty

Resin

5 x 48g

Hardener SOFT

5 x 52g

Accelerator

5 x 2.5ml

 

 

Catalog #

Description

Qty

0653

Taab 812 Premix Kit Hard

1Kit

0654

Taab 812 Premix Kit Medium

1 Kit

0655

Taab 812 Premix Kit Soft

1 Kit

Please use the catalog number and description when ordering.

 
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Lowicryl Resin

 

Lowicryl K4M Kit,polar


Lowicryl resins are highly cross-linked acrylate based embedding resins. These resins provide low viscosity at low temperature. Both Lowicryl K4M and Lowicryl K11M are water miscible and can be used at low temperature for embedding as K4M -35°C and K11M -60°C. This is Very useful in preventing lipid and protein extraction. Both resins are polar (hydrophilic) media.


Lowicryl HM20 and Lowicryl HM23 are nonpolar (hydrophobic) resins which can be used even at temperatures as low as -70°C in the case of  Lowicryl HM20 and -80°C. in the case of  Lowicryl HM23.

Lowicryl K4M is very useful for immuno-staining techniques. Immuno staining with Protein A Coated colloidal gold does not interfere with post-staining on Lowicryl K4M sections. All of these resins are photopolymerized by long wavelength
(360 nm) ultraviolet light. They can also be chemically polymerized at 60° C.
 

Lowicryl Resin Kits

Kit Contents

Each Lowicryl Kit contains sufficient resin, cross linking agent, and catalyst to make approximately 900g


 

Catalog #

Description

Qty

057

Lowicryl K4M Kit,polar

1 Kit

0571

Lowicryl K11M Kit, polar

1 Kit

058

Lowicryl HM20 Kit, non-polar

1 Kit

0581

Lowicryl HM23 Kit, non-polar

1 Kit

Please use the catalog number and description when ordering.


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Lowicryl MonoStep Embedding Media

Single Component


This pre-mixed MonoStep Lowicryl Media is the same as  our Lowicryl complete kit. You do not have to prepare and mix the components. It will allow you to make one step embedding. The Lowicryl MonoStep Media ready to use resin is a real time saver. It is Ideal for use in Immunohistochemistry and immunolabeling.
 

Catalog #

Description

Qty

05810

MonoStep Lowicryl HM20, Non-Polar embedding
medium

225 g

 05710

MonoStep Lowicryl  K4M Polar embedding
medium

225 g

Please use the catalog number and description when ordering.

 

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JB-4™ Embedding Kit

 

The JB-4™ is a water soluble embedding medium based  on Glycol Methacrylate plastic embedding. This is the leading plastic embedding medium for high resolution light microscopy.  Conventional paraffin sections have much greater shrinkage and inadequate morphology when compared to JB-4™.

 

JB-4™ Embedding KitFeatures

 

Easily cuts thin sections, 0.5 - 2 microns

 

Cures at room temperature


Gives sharp and brilliant staining


 Water soluble


Gives good results even  with difficult specimens

 

 

 

JB4™ Embedding Kit

Kit Contents

Solution A

800 ml

Solution B

 30 ml

Catalyst

 12 g

 

Catalog #

Description

Qty

060

     JB-4™ Embedding Kit

1 kit

 060-1

     JB-4™ Solution A 

800 ml

060-3

     JB-4™ Catalyst

12 g

060-2

     JB-4™ Solution B

30 ml

Please use the catalog number and description when ordering.

 

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JB-4 Plus™ Embedding Kit


The JB-4 Plus™ offers all the same features as the  JB-4™

 

 Plus

  • It features a cooler acting accelerator system for
    greater protection of sensitive tissue components

Plus

  • It  yields a crystal clear and harder block which is
    ideal for dense tissue samples

JB4 Plus™ Embedding Kit

Kit Contents

Solution A

500 ml

Solution B

15 ml

Catalyst

8 g

 

Catalog #

Description

Qty

0601

JB-4 Plus™ Embedding Kit

1 kit

0601-1

JB-4 Plus™ Solution A

500 ml

 0601-2

JB-4 Plus™ Solution B

15 ml

0601-3.

JB-4 Plus™ Catalyst

8 g

Please use the catalog number and description when ordering.

 

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Nanoplast FB101 Embedding Kit

DISCONTINUED
Nanoplast FB101 Embedding Kit

 

Nanoplast FB101 is a highly water-soluble melamine resin system for use in electron microscopy. It is particularly suitable for high resolution. Apart from possessing excellent casting properties it permits the infiltration of biological specimens without the need of conventional dehydration with organic solvents.


Biological materials which have been embedded in Nanoplast and cut with a diamond knife yield a structural resolution unattainable by other methods.

 

DISCONTINUED

Nanoplast FB101 Embedding Kit

Kit Contents

Qty

Melamine Resin MME 7002

(hexamethylol-melamine-methyl ether,70% in water)

250 g

Acid Catalyst B-52 (p-toluene sulfonic acid)

10 g

Flat Embedding Mold

1

DISCONTINUED

Nanoplast FB101 Mini Set Kit

Kit Contents

Qty

Melamine Resin MME 7002

(hexamethylol-melamine-methyl ether,70% in water)

50 g

Acid Catalyst B-52 (p-toluene sulfonic acid)

2 g

Flat Embedding Mold

1

 

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Styrene Monomer, Polyester Resin Kit


Very low viscosity polyester resin kit. Monomeric styrene penetrates rapidly into the tissues. The infiltration and embedding can be done by styrene alone. Polymerization can be done by UV light or heat.

 

Styrene Monomer Polyester Resin Kit

Kit Contents

Qty

Monomeric Styrene

450 ml

Methyl Ethyl Ketone

100 ml

Dibutyl Phthalate

225 ml

n-butyl Methacrylate

225 ml

Benzoyl peroxide

20 g

 

Catalog #

Description

Qty

04914

Styrene Monomer, Polyester Resin kit,

1 Kit

Please use the catalog number and description when ordering.

 

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Durcupan™


A water soluble embedding medium for EM.

 

Durcupan

Kit consists of 260 g for a minimum of 10 embedments

Durcupan™ is a registered trade mark of A. Fluka A.G. Switzerland.
 

Catalog #

Description

Qty

 06610

 Durcupan embedding medium kit

1 Kit

Please use the catalog number and description when ordering.

 

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UNICRYL™ Embedding Kit

This new Universal resin embedding media is for:

  • Light and Electron Microscopy

  • Immunolabelling

  • In-Situ Hybridization

  • Histochemistry.

 Unicryl™ is a single component new resin. It is easy to use and provides excellent structural reservation. Unicryl™ is largely hydrophilic, allowing good access to polar solutions. The tissues do not  chemically  interact or crosslink with the resin.


Unicryl™ has excellent polymerization properties which is mainly due too the fact that all of the components of the polymer have similar molecular weights.


Unicryl™ is premixed and is supplied as a single component. Unicryl has a long shelf life when it is stored in the cold. The resin has a low viscosity, even at temperatures as  low as -50°C. It is miscible with alcohol,  ethyl alcohol, acetone and many other solvents that can be used for dehydration.


The Resin can be polymerized by heat: 50°C - 60°C or by UV irradiation at low temperature: -10°C  to -20°C.
 

Unicryl™ Embeding Kit

Kit Contents

Qty

 Unicryl Resin

250 ml

1 ml embedding vials.

1 pkg

Plastic gloves

1 pr

Plastic bag for disposal

1

Waste container

1

 

Catalog #

Description

Qty

067

UNICRYL Embedding Kit

1 Kit

Please use the catalog number and description when ordering.


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UNICRYL Staining Kit


 

 

This staining kit has been designed especially for staining tissues which have been embedded in UNICRYL resin. From the UNICRYL embedded block semi-thin or ultra-thin sections can be easily cut. The sections can be immunolabelled with antibodies, or using the staining kit for chromatic stains. This Kit may be stored for at least 12 months at 4°C

 

UNICRYL Staining Kit

Kit Contents

Qty

Hematoxylin

 100ml

Silver Methenamine

 100ml

Light Green

 100ml

Eosin

 100ml

Safranin

 100ml

Toluidine Blue

 100ml

 

 

Catalog #

Description

Qty

0671

UNICRYL staining kit

1 Kit

Please use the catalog number and description when ordering.

 

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Histocryl Resin Kit

 

Histocryl resin kit

Histocryl is a hydrophilic acrylic resin of low toxicity which is     easy to use and is formulated specifically for light microscopy.   It permits the use of most routine staining techniques without prior removal or etching of the resin.

 

Histocryl Resin Kit

Kit Contents

Qty

Histocryl

500g

Catalyst

50g

Accelerator

10 ml

 

Catalog #

Description

Qty

CH010-0

Histocryl resin kit

1 Kit

Please use the catalog number and description when ordering.

 

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Technovit® Glycol Methacrylate  Embedding Kits

Technovit plastic embedding kits from Heraeus Kulzer in Germany are standard where convenience of use, quality of components and results, and extensive technical heritage are important. Several reasons for the popularity of Technovit®  Include:

  • Technovit® gives better results with improved morphology and plastic makes serial one micron sectioning possible.

  • Technovit® 7100 is less sensitive to Oxygen during polymerization so sealing the molds is not necessary.

  • The polymerization temperature never exceeds 40° C making either Technovit ® kit a potential choice for enzyme histochemistry. Initiation of Technovit® 8100  makes polymerization at 4°C possible for improved  signal during immunohistochemistry.

  • The  initiator is prepackaged thus eliminating messy handling of dry components . By eliminating aromatic amines from the formulation,  the components are significantly less toxic.

These benefits plus rapid delivery from Canemco make Technovit the GMA kit of choice for your work.

 



Technovit® 7100 Glycol Methacrylate
Embedding Kit

 

Technovit 7100 GMA embedding kit

Technovit ® 7100 is the standard GMA kit in Europe today in most laboratories desiring the improved morphology only a plastic section provides.

 

Technovit® 7100 Kit

Kit Contents

Qty

GMA monomer

500 ml

Hardener I

5 x 0.6 g

Hardener II

40 ml

The initiator is packaged
individually

 0.6 g

 

Catalog #

Description

Qty

H7100

Technovit 7100 GMA embedding kit

1 Kit

Please use the catalog number and description when ordering.


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Technovit® 8100 Glycol Methacrylate
Embedding Kit


Technovit® 8100 has been specially formulated for low temperature polymerization at 4°C. This combination of enhanced signal for immunohistochemistry and improved morphology from a plastic section makes Technovit® 8100 the choice for your most sensitive applications.
 

Technovit® 8100 Kit

Kit Contents

Qty

GMA monomer

500 ml

Hardener I

5 x 0.6 g

Hardener II

30 ml

The initiator is packaged
individually

 0.6 g

Cover foils for 500 embedments

 

Catalog #

Description

Qty

H8100

Technovit® 8100 GMA embedding kit

1 Kit

Please use the catalog number and description when ordering.

 

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Technovit® 9100 Methyl Methacrylate
Embedding Kit

 

Technovit 9100 MMA embedding kitTechnovit® 9100 is a polymerization system based on methyl methacrylate (MMA) which hardens at low temperature. It has been specially developed for embedding mineralized tissues with extensive possibilities of staining for light microscopy.


The chemical polymerization of Technovit® 9100 takes place under the exclusion of oxygen with the aid of a catalyst composed of a peroxide and an amine components such as PMMA powder and a regulator allow for a controlled polymerization at temperatures between -8°C and -20°C, which guarantees a dispersion of the heat generated during polymerization. Polymerization time is between 18 - 24 hours when using a volume between 3 -15 ml in the above temperature range.
 

Technovit ® 9100 kit

Kit Contents

Qty

Basic Solution (stabilized)

1000ml

PMMA-Powder

120g

Hardener 1.

 8 x 1 g

Hardener 2.

10 ml

Polymerization regulator

5 ml

PMMA-Granulate

500g

 

Catalog #

Description

Qty

H9100

Technovit 9100 MMA embedding kit

1 Kit

Please use the catalog number and description when ordering.


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Technovit® 3040

 

Technovit® 3040 KitTechnovit® 3040 is a yellow,  fast curing methyl methacrylate based resin. Its chemical composition warrants a firm durable bond with Technovit® 7100 or Technovit® 8100 and secure fixing of the specimens to the histobloc. Technovit® 3040 consists of two components, (power and liquid), allowing easy mixing, good adherence to the specimen, and fast curing. Technovit® 3040 should be mixed to a highly viscous consistency. Depending on the room temperature, Technovit® 3040 will cure in 5-10 minutes.

 

 

Technovit® 3040 Kit

Contents

Qty

Technovit® 3040 powder

100 g

Technovit ® 3040 liquid

80 ml

 

Catalog #

Description

Qty

H3040

Technovit® 3040 Mounting medium

1 kit

Please use the catalog number and description when ordering.


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Immuno-Bed™Embedding Kit

 

Low viscosity media for light microscopy and immunohistochemistry techniques.

 

Immuno-Bed™ Embedding Kit

Kit Contents

Qty

Solution A

800 ml

Solution B

30 ml

Catalyst

12 g

 

Catalog #

Description

Qty

19934

Immuno-Bed embedding kit

1 kit

Please use the catalog number and description when ordering.


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Online Catalogs

catalog

For your convenience we have also provided our latest supplemental catalogs in PDF format here.

Other catalogs may be found within the Products section.